Download original image
Fig. 1.

Immunolocalization of ZO-1 (green), Cldn1, and 4 (red) in the adult epididymis. (A) A pig epididymis section labeled with anti-ZO-1. Tight junctions were observed at the apical regions between epithelial cells (yellow arrowhead). (B) A pig epididymis section labeled with anti-Cldn1. Expression of Cldn1 was observed at basolateral (white arrows) and basement membranes (white arrowheads). (C) A pig epididymis section labeled with anti-Cldn4. Expression of Cldn4 was observed at basolateral (white arrows) and basement membranes (white arrowheads). (D-F) Negative controls included staining with secondary antibody only and showed no staining of ZO-1, Cldn1, and Cldn4 in the epididymis. Nuclei are labeled with DAPI (blue). P, principal cells; B, basal cells; L, lumen; S, spermatozoa. Bars = 50 mm.

Journal of Animal Reproduction and Biotechnology 2019;34:190~196 https://doi.org/10.12750/JARB.34.3.190
© J Anim Reprod Biotechnol