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Fig. 3.

Double immunostaining for Cldn4 (red) and ZO-1 (green, yellow arrowheads) in the pig epididymis. Cldn4 was punctate along the basolateral membranes in the caput (A, A’; white arrowhead) and corpus (B, B’; white arrowhead) regions of the epididymis. Nuclei are labeled with DAPI (blue). P, principal cells; B, basal cells; L, lumen; S, spermatozoa. Bars = 50 mm. A 3D reconstruction of TJs double labeled for ZO-1 (yellow arrowhead) and Cldn4 (white arrows). No colocalization of Cldn1 and ZO-1 was detected in the entire epididymis (D). A Significant increase in Cldn4 expression was observed at the cauda compared to caput and corpus regions of the epididymis (E). Data are represented as means ± SEM. *p < 0.01.

Journal of Animal Reproduction and Biotechnology 2019;34:190~196
© J Anim Reprod Biotechnol