Table. 1.

Bioactivity of eelFSH receptors in cells expressing activating receptor mutants

 eelFSH receptorscAMP responses

Basal (nM/104 cells)EC50 (ng)Rmax (nM/104 cells)
eelFSHR-WT (2.5 ug)1.0 ± 0.3 (1-fold)538.5 (100%)40.3 ± 2.8 (100%)
eelFSHR-D540G (2.5 ug)13.6 ± 0.5 (13.1-fold)555.5 (15%)27.6 ± 4.3 (68.5%)
eelFSHR-WT (5 ug)1.0 ± 0.2 (19.1-fold)543.8 (100%)61.3 ± 4.7 (100%)
eelFSHR-D540G (5 ug)19.6 ± 2.3 (19.6-fold)685.0 (20%)50.8 ± 3.5 (82.9%)

Values are the means ± SEM of triplicate experiments. EC50 values were determine determined from the concentration-response curves from in vitro bioassays.

Journal of Animal Reproduction and Biotechnology 2019;34:267~271 https://doi.org/10.12750/JARB.34.4.267
© J Anim Reprod Biotechnol