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Fig. 2.

Basal and FSH-stimulated cAMP production by CHO-K1 cells transiently transfected with wild type (o) or D540G mutated (•) FSH receptor constructs. Left (A): transfection with 2.5 μg DNA plasmid. Right (B): transfected with 5 μg DNA plasmid. cAMP production in response to increasing concentrations of recombinant-eelFSH. Results are expressed as the mean ± SEM of two individual experiments performed in duplicate. Basal cAMP was measured by transfecting the cells with pcDNA3 vectors.

Journal of Animal Reproduction and Biotechnology 2019;34:267~271
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