Immunohistological expression of CYP1A2 in prepubertal and pubertal ovaries. The negative expressions of CYP1A2 at the primordial, primary and secondary follicles in both groups (red arrows in A and D). The CYP1A2-positive cells, localized at the granulosa cells, from antral follicles (blue arrows in Fig. B and E) to pre-ovulatory follicles (blue arrows in C and F). Magnification: ×400 (A, B, D and E) or ×100 (C and F); Bars: 100 μm (A, B, D and E) or 1,000 μm (C and F).