Apoptotic patterns in blastocysts derived from GD1a and GT1b treated embryos. Total chromatin content were determined by staining with DAPI (blue) and fragmented DNA was labeled via terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL; green, white arrows). Merged images show TUNEL and DAPI signals. Scale bar = 100 µm. Data in the bar graph represent the means ± SEM of three independent experiments. Differences were considered significant at *p < 0.05 and **< 0.01.