Detection of ROS levels in blastocysts derived from ganglioside GD1a and GT1b treated embryos by H2DCFDA staining. (A) Fluorescence imaging of ROS production in blastocysts by H2DCFDA. (B) Quantification of ROS levels in blastocysts. Scale bar = 100 µm. Data in the bar graph represent the means ± SEM of three independent experiments. Differences were considered significant at ***p < 0.001.