Cumulus expansion by alphalinolenic acid (ALA; 50 μM) in porcine oocytes at 22 h after in vitro maturation in porcine oocytes. (A) Microscopic images of expanded cumulus layer. (B) Relative value of cumulus area normalized to control group. Scale bar: 200 μm. A,BDifferent superscripts indicate significant difference between 0 h and 22 h in ALA group after in vitro maturation (p < 0.05). a,bDifferent superscripts indicate significant difference between 0 h and 22 h in control group after in vitro maturation (p < 0.05). Asterisks indicate differences between control group and ALA group at 22 h after in vitro maturation (p < 0.05).
