Table. 1.

Effects of alpha-linolenic acid (ALA; 50 μM) during in vitro maturation on nuclear maturation in porcine oocytes

Period of in vitro maturation (h) Addition of ALA No. of oocytes examined No. (%) of oocytes matured to

GV GVBD MI AI TI MII
0 162 162 0 0 0 0 0
(100.0 ± 0.0) (0.0 ± 0.0) (0.0 ± 0.0) (0.0 ± 0.0) (0.0 ± 0.0) (0.0 ± 0.0)
22 − 158 25 19 100 2 12 0
(15.0 ± 3.7)b (13.1 ± 4.6) (62.9 ± 2.7)a (1.1 ± 1.1) (8.0 ± 2.2) (0.0 ± 0.0)
+ 171 7 13 144 0 7 0
(4.0 ± 2.2)a (8.3 ± 3.5) (83.5 ± 2.7)b (0.0 ± 0.0) (4.2 ± 1.3) (0.0 ± 0.0)
44 − 161 2 4 35 2 1 117
(1.0 ± 0.6) (2.2 ± 0.9) (22.0 ± 1.7)b (1.0 ± 1.0) (0.6 ± 0.6) (73.1 ± 2.1)a
+ 179 3 3 9 0 0 164
(1.7 ± 1.1) (1.7 ± 0.6) (5.0 ± 0.9)a (0.0 ± 0.0) (0.0 ± 0.0) (91.6 ± 0.7)b

Data were presented as number (means ± SEM) from four replications.

a,bDifferent superscripted letters indicate a significant difference in the same time (p < 0.05).

GV : germinal vesicle; GVBD : germinal vesicle break down; MI : metaphase I; AI : anaphase I; TI : telophase I; MII : metaphase II.

Journal of Animal Reproduction and Biotechnology 2020;35:357-65 https://doi.org/10.12750/JARB.35.4.357
© 2020 Journal of Animal Reproduction and Biotechnology
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