Journal of Embryo Transfer 2007; 22(3): 179-184
Published online September 29, 2007
Copyright © The Korean Society of Animal Reproduction and Biotechnology.
김혜민, 이상미, 박효영, 문승주, 강만종
Culture method of somatic cells is one of the important factors in the production of transgenic pigs by somatic cell nuclear transfer. In this study, we established an efficient culture method of porcine fetal fibroblasts. Porcine fetal fibroblasts were isolated from 33-day-old fetuses. The proliferation of porcine fetal fibroblasts was analyzed by different serum types and culture media. The cultures in medium supplied 15% ES screened FBS showed faster increase in cell number than 15% FBS. Also, fetal fibroblasts have been propagated continuously for
Keywords: cell culture, porcine, transfection, fetal fibroblast