Journal of Embryo Transfer 2010; 25(4): 259-262
Published online December 31, 2010
Copyright © The Korean Society of Animal Reproduction and Biotechnology.
Park, Sang-Kyu, Choi, Young-Ju, Roh, Sang-Ho
Cellular Reprogramming and Embryo Biotechnology Laboratory, Dental Research Institute and CLS21, Seoul National University School of Dentistry, Seoul 110-749, Korea
Optimization of the preimplantation mammalian embryo culture condition was widely focused on refining medium composition under the name of chemically defined media. However, recent research revealed that the alteration of physical environment can be a crucial factor to a successful embryo development. In this study, under the same embryo density, a novel culture device named oil-free micro tube culture (MTC) system was evaluated using porcine parthenogenetic embryos. The activated oocytes were placed into the 0.2 ml thin-wall flat cap PCR tube and cultured to the blastocyst stage. As a preliminary step, embryo density and culture medium volume were optimized under a standard drop culture system. The optimal embryo density range for in vitro culture was 0.5 embryos per
Keywords: oil-free, microtube culture, porcine, parthenogenesis