Journal of Embryo Transfer 2012; 27(3): 183-187
Published online September 30, 2012
Copyright © The Korean Society of Animal Reproduction and Biotechnology.
국립축산과학원 바이오공학과, 국립경상대학교 수의과대학 수의산과학연구실
Adult stem cell transplantation has been increased every year, because of the lack of organ donors for regenerative medicine. Therefore, development of reliable and safety cryopreservation and bio-baking method for stem cell therapy is urgently needed. The present study investigated safety of dimethyl sulfoxide (DMSO) such as common cryoprotectant on porcine bone marrow derived mesenchymal stem cells (pBM-MSCs) by evaluating the activation of Caspase-3 and -7, apoptosis related important signal pathway. pBM-MSCs used for the present study were isolated density gradient method by Ficoll-Paque Plus and cultured in A-DMEM supplemented 10% FBS at
Keywords: porcine, mesenchymal stem cells, apoptosis, cryopreservation, DMSO, caspase-3 and -7