JARB Journal of Animal Reproduction and Biotehnology

OPEN ACCESS pISSN: 2671-4639
eISSN: 2671-4663

Article Search

Article
Split Viewer

Journal of Embryo Transfer 2008; 23(2): 87-91

Published online June 30, 2008

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

Effects of Glutamine, Glucosamine and Glutathione on the In Vitro Maturation of Porcine Oocytes

Kim, Young-Hee, Rhee, Man-Hee, Kim, Sang-Keun

College of Veterinary Medicine, Chungnam National University, Daejeon 305-764, Korea

Abstract

This study was carried out to investigate the effects of the supplementation of glutamine, glucosamine and glutathione on the porcine oocytes on IVM rates. Cocs were incubated in NCSU-23 supplemented with at $2.0{sim}10.0;mM$ glucosamine, $0.5{sim}4.0;mM$ glutamine and $0.1{sim}1.0;mM$ glutathione for 48 hrs. Oocytes were transferred to 50 ul drops of maturation medium covered with mineral oil and cultured in a $CO_2$ incubator ($38^{circ}C$, 5% $CO_2$, 95% air). The IVM rates of oocytes cultured in NCSU-23 supplemented with 0.5, 1.0, 2.0 and 4.0 mM glutamine for 48 hrs were $46.0{pm}4.5%$, $52.0{pm}4.8%$, $50.0{pm}4.2%$ and $44.0{pm}4.5%$, respectively. The IVM rates of oocytes cultured in NCSU-23 supplement with 2.0, 5.0, 7.0, 10.0 mM glucosamine for 48 hrs were $44.0{pm}4.5%$, $42.0{pm}4.5%$, $38.0{pm}4.6%$ and $24.0{pm}4.8%$, respectively. The IVM rates of oocytes cultured in NCSU-23 supplemented with glucosamine were no significantly increased compare to the control ($42.5{pm}4.0%$). The IVM rate of oocytes cultured in NCSU-23 supplemented with 3.0, 5.0, 7.0, 10.0 mM glutathione for 48 hrs were $40.0{pm}3.2%$, $54.0{pm}4.2%$, $48.0{pm}4.5%$, $44.0{pm}4.8%$, respectively. The IVM rate of oocytes cultured in NCSU-23 supplemented with glutamine and glutathione were significantly increased co~pared to those control ($42.5{pm}4.0%$). Glucosamine did not affect the IVM rates of oocytes. IVM rates of oocytes cultured in NCSU-23 medium for 48 hrs were significantly increased compared to the cultured for 40 hrs.

Keywords: porcine oocytes, glucosamine, glutamine, glutathione, IVM rate

Article

Journal of Embryo Transfer 2008; 23(2): 87-91

Published online June 30, 2008

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

Effects of Glutamine, Glucosamine and Glutathione on the In Vitro Maturation of Porcine Oocytes

Kim, Young-Hee, Rhee, Man-Hee, Kim, Sang-Keun

College of Veterinary Medicine, Chungnam National University, Daejeon 305-764, Korea

Abstract

This study was carried out to investigate the effects of the supplementation of glutamine, glucosamine and glutathione on the porcine oocytes on IVM rates. Cocs were incubated in NCSU-23 supplemented with at $2.0{sim}10.0;mM$ glucosamine, $0.5{sim}4.0;mM$ glutamine and $0.1{sim}1.0;mM$ glutathione for 48 hrs. Oocytes were transferred to 50 ul drops of maturation medium covered with mineral oil and cultured in a $CO_2$ incubator ($38^{circ}C$, 5% $CO_2$, 95% air). The IVM rates of oocytes cultured in NCSU-23 supplemented with 0.5, 1.0, 2.0 and 4.0 mM glutamine for 48 hrs were $46.0{pm}4.5%$, $52.0{pm}4.8%$, $50.0{pm}4.2%$ and $44.0{pm}4.5%$, respectively. The IVM rates of oocytes cultured in NCSU-23 supplement with 2.0, 5.0, 7.0, 10.0 mM glucosamine for 48 hrs were $44.0{pm}4.5%$, $42.0{pm}4.5%$, $38.0{pm}4.6%$ and $24.0{pm}4.8%$, respectively. The IVM rates of oocytes cultured in NCSU-23 supplemented with glucosamine were no significantly increased compare to the control ($42.5{pm}4.0%$). The IVM rate of oocytes cultured in NCSU-23 supplemented with 3.0, 5.0, 7.0, 10.0 mM glutathione for 48 hrs were $40.0{pm}3.2%$, $54.0{pm}4.2%$, $48.0{pm}4.5%$, $44.0{pm}4.8%$, respectively. The IVM rate of oocytes cultured in NCSU-23 supplemented with glutamine and glutathione were significantly increased co~pared to those control ($42.5{pm}4.0%$). Glucosamine did not affect the IVM rates of oocytes. IVM rates of oocytes cultured in NCSU-23 medium for 48 hrs were significantly increased compared to the cultured for 40 hrs.

Keywords: porcine oocytes, glucosamine, glutamine, glutathione, IVM rate