JARB Journal of Animal Reproduction and Biotehnology

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eISSN: 2671-4663

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Journal of Embryo Transfer 2010; 25(1): 67-75

Published online March 31, 2010

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

시츄견에서 질세포 검사를 이용한 발정 유기 효과의 확인

박철호, 이숙경, 김방실, 배재한, 류재선, 김상일, 이주환, 박상국, 박인철, 김종택, 서국현, 오기석, 손창호

전남대학교 수의과대학

Abstract

To investigate the change of vaginal epithelial cell in estrus-induced Shih-tzu bitch, estrus was induced by PMSG (50 IU/kg, for 10 days) and hCG (1,000 IU, on Day 10) in 12 anestrus Shih-tzu bitches. Day-changes of vaginal epithelial cells during the whole period of hormone treatment were investigated in each experimental bitches. The first day of vulvar bleeding and the first day of male acceptant was on $6.72{pm}1.38$ days ($Mean{pm}S.D.$), and $10.44{pm}1.63$ days after the first PMSG treatment. The duration of proestrus and estrus was $4.36{pm}1.20$ days, and $7.58{pm}1.43$ days. Characteristic features of vaginal cytology during the estrous cycle were the high proportion of large intermediate cell, superficial cell, anuclear cell and erythrocyte in proestrus, superficial cell and anuclear cell in estrus, and parabasal cell, small and large intermediate cell, and leukocyte in diestrus, respectively. When it was timed from the first day of PMSG administration (Day 0), the cornification index (CI) was the high proportion in proestrus and estrus. The CI peak was maintained above 80% between Day 11~14 (4 days) and CI showed a peak at Day 12. These results indicated that the 12 Shih-tzu bitches showed positive estrus induction by vaginal smear test and observation of clinical estrus sign.

Keywords: vaginal cytology, estrus induction, estrous cycle, Shih-tzu

Article

Journal of Embryo Transfer 2010; 25(1): 67-75

Published online March 31, 2010

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

시츄견에서 질세포 검사를 이용한 발정 유기 효과의 확인

박철호, 이숙경, 김방실, 배재한, 류재선, 김상일, 이주환, 박상국, 박인철, 김종택, 서국현, 오기석, 손창호

전남대학교 수의과대학, 전라남도 축산기술연구소, 전남대학교 수의과대학, 전남대학교 수의과대학, 전남대학교 수의과대학, 전남대학교 수의과대학, 전남대학교 수의과대학, 전라남도 축산기술연구소, 강원대학교 수의(학부)대학, 강원대학교 수의(학부)대학, 전남대학교 수의과대학, 전남대학교 수의과대학, 전남대학교 수의과대학

시츄견에서 질세포 검사를 이용한 발정 유기 효과의 확인

박철호, 이숙경, 김방실, 배재한, 류재선, 김상일, 이주환, 박상국, 박인철, 김종택, 서국현, 오기석, 손창호

전남대학교 수의과대학

Abstract

To investigate the change of vaginal epithelial cell in estrus-induced Shih-tzu bitch, estrus was induced by PMSG (50 IU/kg, for 10 days) and hCG (1,000 IU, on Day 10) in 12 anestrus Shih-tzu bitches. Day-changes of vaginal epithelial cells during the whole period of hormone treatment were investigated in each experimental bitches. The first day of vulvar bleeding and the first day of male acceptant was on $6.72{pm}1.38$ days ($Mean{pm}S.D.$), and $10.44{pm}1.63$ days after the first PMSG treatment. The duration of proestrus and estrus was $4.36{pm}1.20$ days, and $7.58{pm}1.43$ days. Characteristic features of vaginal cytology during the estrous cycle were the high proportion of large intermediate cell, superficial cell, anuclear cell and erythrocyte in proestrus, superficial cell and anuclear cell in estrus, and parabasal cell, small and large intermediate cell, and leukocyte in diestrus, respectively. When it was timed from the first day of PMSG administration (Day 0), the cornification index (CI) was the high proportion in proestrus and estrus. The CI peak was maintained above 80% between Day 11~14 (4 days) and CI showed a peak at Day 12. These results indicated that the 12 Shih-tzu bitches showed positive estrus induction by vaginal smear test and observation of clinical estrus sign.

Keywords: vaginal cytology, estrus induction, estrous cycle, Shih-tzu