JARB Journal of Animal Reproduction and Biotehnology

OPEN ACCESS pISSN: 2671-4639
eISSN: 2671-4663

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Reproductive & Developmental Biology 2004; 28(2): 113-117

Published online June 1, 2004

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

PZM 배양액이 돼지체외수정란의 배발달에 미치는 영향

한만희,천행수,김종화,박병권,서길웅,이규승

축산연구소,충남대학교 동물자원학,공주대학교 영상보건대학 특수동물학

Abstract

The present study was carried out to examine the effects of O₂ concentrations and culture media (North Carolina State University (NCSU)-23, porcine zygote medium(PZM)-3 or PZM-4) on in vitro development of porcine IVM/IVF embryos. Porcine oocyte-cumulus complexes were cultured in BSA-free NCSU-23 medium containing porcine follicular fluid (10%), cysteine (0.9 mM), β-mercaptoethanol (25 ㎍/㎖), epidermal growth factor (10 ng/㎖) and hormonal supplements (PMSG and hCG: 10 IU/㎖) for 20∼22 h. They were then cultured in the same medium but without hormonal supplements for an additional 20∼22 h. After culture, cumulus-free oocyte were coincubated with liquid boar spermatozoa for 5∼6h. Putative zygotes were transferred to NCSU-23, PZM-3 and PZM-4 medium under the condition of 5% O₂ or 20% O₂ concentrations. At 48 h, no mean differences were found in cleavage rates. However, the rates of blastocyst formation at day 7 after in vitro fertilization were significantly higher in PZM-3 medium under the condition of 5% O₂ concentration than other treatments (19.9±2.4 vs. 11.1±2.0 to 16.0±2.5%, P<0.05). The total cell numbers of blastocysts were significantly higher in 5% O₂ than in 20% O2 (P<0.05). However, no differences was found among the culture media within each O₂ concentrations. In conclusion, the use of PZM-3 medium in 5% O₂ concentration was effective on in vitro development of porcine IVM/IVF embryos.

Keywords: Porcine IVM/IVF embryo, PZM-3, O₂ concentration

Article

Reproductive & Developmental Biology 2004; 28(2): 113-117

Published online June 1, 2004

Copyright © The Korean Society of Animal Reproduction and Biotechnology.

PZM 배양액이 돼지체외수정란의 배발달에 미치는 영향

한만희,천행수,김종화,박병권,서길웅,이규승

축산연구소,충남대학교 동물자원학,공주대학교 영상보건대학 특수동물학

Abstract

The present study was carried out to examine the effects of O₂ concentrations and culture media (North Carolina State University (NCSU)-23, porcine zygote medium(PZM)-3 or PZM-4) on in vitro development of porcine IVM/IVF embryos. Porcine oocyte-cumulus complexes were cultured in BSA-free NCSU-23 medium containing porcine follicular fluid (10%), cysteine (0.9 mM), β-mercaptoethanol (25 ㎍/㎖), epidermal growth factor (10 ng/㎖) and hormonal supplements (PMSG and hCG: 10 IU/㎖) for 20∼22 h. They were then cultured in the same medium but without hormonal supplements for an additional 20∼22 h. After culture, cumulus-free oocyte were coincubated with liquid boar spermatozoa for 5∼6h. Putative zygotes were transferred to NCSU-23, PZM-3 and PZM-4 medium under the condition of 5% O₂ or 20% O₂ concentrations. At 48 h, no mean differences were found in cleavage rates. However, the rates of blastocyst formation at day 7 after in vitro fertilization were significantly higher in PZM-3 medium under the condition of 5% O₂ concentration than other treatments (19.9±2.4 vs. 11.1±2.0 to 16.0±2.5%, P<0.05). The total cell numbers of blastocysts were significantly higher in 5% O₂ than in 20% O2 (P<0.05). However, no differences was found among the culture media within each O₂ concentrations. In conclusion, the use of PZM-3 medium in 5% O₂ concentration was effective on in vitro development of porcine IVM/IVF embryos.

Keywords: Porcine IVM/IVF embryo, PZM-3, O₂ concentration