Epifluorescence photomicrographic images of in vitro matured bovine oocytes. Oocytes were stained with Cell Tracker Blue (a-d) and 2’, 7’‑dichlorodihydrofluorescein diacetate (H2DCFDA) (e-h) to detect intracellular levels of glutathione (GSH) and reactive oxygen species (ROS), respectively, whereas, (a and e) control and (b and f) aLA 5mM and (c and g) aLA 10mM and (d and h) aLA 20mM oocytes. Note that, cumulus cells and other substances are not considered (negligible).

Journal of Embryo Transfer 2017;32:123-30 https://doi.org/10.12750/JET.2017.32.3.123