Morphology and germline stem cell marker gene expression of the cell populations used in this study. (A) Cell morphology of em- bryonic cell lines (ECs) established. Each EC was derived from an embryo at stage 32 to 36. ECs were used as feeder cells for culture of enriched ovarian cell populations of Oryzias latipes after subculture over 30 times. In EC1 and EC3, fibroblast-like cells were predominant, while the majority of cells in EC2 were epithelial-like cells. Scale bar = 50 µm. (B) RT-PCR analysis of germline cell marker gene expression. The expression of germline stem cell-specific Nanos2 and germline cell-specific Vasa was detected in total ovarian cells and enriched ovarian cells but not in all ECs.
pISSN: 2671-4639
