Table 1

Primers for SQ-PCR analysis of cell survival and apoptosis associated genes

No.	Name	Sequence
1	GAPDH	Forward	5′-CCC GTT CGA CAG ACA GCC GTG-3′
		Reverse	5′-CCG CCT TGA CTG TGC CGT GG-3′
2	E2-r	Forward	5′-ACA AGC GCC AGA GAG AGA TGA T-3′
		Reverse	5′-AGG ATC TCT AGC CAG GCA CA-3′
3	EGF	Left	5′-CCA GGG GTC TCT TCT CCA AG-3′
		Right	5′-GGT GGG AAT GTA AAC TGG TA-3′
4	IGF	Left	5′-CTC TTC GCA TCT CTT CTA C-3′
		Right	5′-CAA ATG TAC TTC CTT CTG AG-3′
5	AKT1	Forward	5′-GGT CAT GTA CGA GAT GAT GTG CGG C-3′
		Reverse	5′-CTT GAA CGG TGG GCT GAG CTT CTT C-3′
6	mTOR	Forward	5′-CTT TGT CCA GAC CAT GCA GCA GC-3′
		Reverse	5′-TCG TTG ATG CCC TGT AGG TTC AGC-3′
7	PCNA	Forward	5′-GCA CTG AGG TAC CTG AAC TT-3′
		Reverse	5′-TCT TCA TCC TCG ATC TTG GG-3′
8	BCL-2	Forward	5′-CCA GGC CGG CGA TGA CTT CTC T-3′
		Reverse	5′-ATC TCC CGG TTG ACG CTC TCC ACA-3′
9	BAX	Forward	5′-ATG GAC GGG TCC GGG GAG CAA-3′
		Reverse	5′-TCA GCC CAT CTT CTT CCA GAT-3′
10	20a-HSD	Forward	5′-ACC ACA GTC CAT GCC ATC AC-3′
		Reverse	5′-TCC ACC ACC CTG TTTG CTG TA-3′
11	Casp-3	Forward	5′-CAT GGT CAG GCC TTG TGA AGC TGA-3′
		Reverse	5′-TCT TCT TCA TGA CCT GAC CGT CGG-3′

Journal of Animal Reproduction and Biotechnology 2020;35:163-70 https://doi.org/10.12750/JARB.35.2.163