Comparison of survival rate, intracellular ROS production, and apoptosis level in vitrified-warmed bovine blastocysts obtained using Cryotop and ReproCarrier products. (A) Morphology of vitrified-warmed bovine blastocysts visualized using microscope. (B) The survival rates of vitrified-warmed bovine blastocysts using Cryotop and ReproCarrier. (C) Vitrified-warmed bovine blastocysts using Cryotop and ReproCarrier were evaluated for intracellular ROS (green) by H2DCFDA staining. Scale bar = 100 µm. Data in the bar graph represent means ± SD from three independent experiments. (D) Colocalization of DAPI and TUNEL stains at the blastocyst stage. The blastocysts were stained for chromosome (blue) and fragmented DNA (green). The white arrows indicate a merged spot with chromosome and fragmented DNA. Data in the bar graph represent the means ± SD from three independent experiments. Scale bar = 200 µm.
pISSN: 2671-4639
