Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and propidium iodide (PI) staining of blastocyst stage embryos cloned with tMSC (I), eMSC (II) and sMSC (III). (A) Representative fluorescent microscope images. Apoptotic bodies and nuclei were labeled with TUNEL staining (white arrows) and PI (red), respectively. Scale bar = 50 μm. (B) Total cell number. (C) Apoptotic incidence. Graphs were presented as mean ± SEM. tMSC: telomerase reverse transcriptase-transfected MSC, sMSC: sham-transfected MSC, eMSC: early passage MSC. Asterisk indicates significant difference (p < 0.05). 4 replicates.
pISSN: 2671-4639
