Comparison of the efficiency of metaphase spread preparation from Haliotis discus hannai larvae between two different developmental stages. Metaphase spreads were prepared from diploid or triploid larvae at trochophore or veliger stage and the ratios of metaphase spreads out of total cells were measured. Percentage of metaphase spreads was significantly higher in trochophore compared to veliger stage larvae regardless of ploidy status. All values are expressed as mean ± standard deviation of five independent experiments. abDifferent letters indicate significant differences (p < 0.05).