Table 1

Sequences and condition of primer sequence used to real-time PCR

Gene	Primer sequence (5’ → 3’)	Product size (bp)	Accession number
Transforming growth factor-beta 1 (TGF- β 1)	F: ACCTGCAAGACCATCGACAR: GCTCCGGTTCGACACTTTCC	196	NM_214015.2
Transforming growth factor-beta 2 (TGF- β 2)	F: GCGCTACATCGACAGCAAAGR: TGCAGCAGGGACAGTGTAAG	143	XM_021064293.1
Transforming growth factor-beta 3 (TGF- β 3)	F: CTGTGCGTGAATGGCTCTTGR: CTTAAGTCGCCCCAGGTCTC	185	NM_214198.1
Transforming growth factor-beta receptor 1 (TGF- β R1)	F: AAGCCACCATCTGGCCTTGR: TGGCACTCGATGGTGAATGA	132	NM_001038639.1
Transforming growth factor-beta receptor 1 (TGF- β R2)	F: TGGCTGTCTGGAGGAAGAATGR: ATCCGTGGTAGGCGATCTTG	78	XM_021071493.1
Urokinase-type plasminogen activator (uPA)	F: TGCATCGAACTGTGGCTGTCR: GACCGTTCCCCTCAAAGCAG	146	NM_213945
Tissue-type plasminogen activator (tPA)	F: CAGCCGCTGCACTTCAAAACR: GTGTACACACCCGGAACGTC	222	NM_214054
Urokinase-type plasminogen activator receptor (uPAR)	F: GCACACATGGGAAGGAGGTGR: CGAGGCACAGGAAGCACATT	214	XM_003127198
Plasminogen activators inhibitor type-1 (PAI-1)	F: CACCGCCTCTTCCACAAGTCR: ATGCTCAGAGTGTTGCCGTG	149	NM_213910
Interleukin-6 (IL-6)	F: ACAAAGCCACCACCCCTAACR: CGTGGACGGCATCAATCTCA	185	NM_214399.1
Interleukin-8 (IL-8)	F: AGTGCAGAACTTCGATGCCAR: TGGGGTCCACTCTCAATCAC	95	NM_213867.1
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)	F: CATGGTTCACGCCCATCACAR: TCCCGCCAACATCAAATGGG	170	NM-001206359

Journal of Animal Reproduction and Biotechnology 2020;35:297-306 https://doi.org/10.12750/JARB.35.4.297