JARB Journal of Animal Reproduction and Biotehnology

OPEN ACCESS pISSN: 2671-4639
eISSN: 2671-4663

Table. 3.

Viability, acrosomal membrane integrity, and mitochondrial membrane potential of spermatozoa after freezing-thawing

Straw size (mL) Replicates LIAH LIAL DIAL DDAL
0.25 25 48.0 ± 2.6c 8.4 ± 0.7f 23.1 ± 2.0 18.6 ± 1.6
0.5 25 35.6 ± 2.8d 16.6 ± 1.8e 25.7 ± 2.1 20.4 ± 1.2

c,dValues (mean ± SE) with different superscripts in the same column are significantly different (p < 0.01). e,fValues (mean ± SE) with different superscripts in the same column are significantly different (p < 0.001). Values indicate mean of 25 replicates (5 replicates × 5 ejaculates). Spermatozoa with live intact acrosome and high mitochondrial membrane integrity, LIAH; spermatozoa with live intact acrosome and low mitochondrial membrane integrity, LIAL; spermatozoa with dead intact acrosome and low mitochondrial membrane integrity, DIAL; spermatozoa with dead damaged acrosome and low mitochondrial membrane integrity, DDAL; five straws of frozen-thawed semen were used to evaluate spermatozoa characteristics per ejaculation group.
Journal of Animal Reproduction and Biotechnology 2020;35:307-14 https://doi.org/10.12750/JARB.35.4.307