Detection of ROS in dimethachlor-exposed embryos. (A) ROS levels increased in the anterior region. White arrows indicate regions where ROS levels markedly increased. Scale bar: 500 μm. (B) Green fluorescence intensity of DCFH-DA was quantified using “RawIntDen” in ImageJ software and is displayed as a percentage relative to the solvent group. p-values that indicate significant differences: **p < 0.01 and ***p < 0.001. DCFH-DA, dichloro-dihydro-fluorescein diacetate; ROS, reactive oxygen species.