Isoprocarb toxicity in developing zebrafish embryos. (A) Pathological alterations revealed abnormal embryonic development in zebrafish larvae at 72 hpf. The transparent body of the zebrafish embryo-larvae facilitates visualizing internal development. As compared with controls, distorted phenotypic changes including inflation of the heart and yolk sac to an unusual level, spinal curvature, and reduction in overall body length were detected (highlighted by colored box; heart - red and yolk sac - blue, line; curved body axis - purple). Scale bar: 300 μm. (B-D) Quantitative values of toxicity endpoints supporting those observed defects - (B) body length, (C) yolk edema, and (D) heart edema (***p < 0.001).