Detection of apoptotic cell death in zebrafish larvae using acridine orange staining. (A) Evidence of developmental defects - acridine orange-positive apoptotic cells were highly increased in the eye and brain in the anterior part, and a significant number of green fluorescent cells were also observed in the curved tail in the posterior part of the body. Scale bar: 100 μm. (B) The number of apoptotic cells with each concentration of isoprocarb was counted at 72 h of development in the individual larva (*p < 0.05 and ***p < 0.001).
pISSN: 2671-4639
