Effect of chronic peripheral administration of kisspeptin on feed intake and body weight. Saline or kisspeptin was administered from day 25 to day 50. (A) Cumulative feed intake calculated from feed consumed by 2-3 rats housed in cages over the experimental period and averaged per rat. Control vs. kisspeptin treated group: p < 0.001, two-way ANOVA. (B) Average body weight. Control vs. kisspeptin treated group : p < 0.001, two-way ANOVA. (C) Weight of gonads (uterus + ovaries), heart and liver. Control vs. kisspeptin treated group in heart weight: p = 0.03, t-test. Data expressed as mean ± SEM.