Effect of chronic peripheral administration of kisspeptin on plasma LH and oestradiol concentration. Saline or kisspeptin (100 nmol i.p) was administered from day 25 to day 50. On day 66, the animals were again treated with either saline or kisspeptin (100 nmol), i.p., and after an hour, blood was collected retro-orbitally to study LH and oestradiol response. There was no difference among the groups in plasma LH (A) and oestradiol (B) concentrations. Data expressed as mean ± SEM.