Effect of chronic peripheral administration of kisspeptin on GPR54 in gonads. Saline or kisspeptin (100 nmol i.p) was administered from day 25 to day 50. On day 66, the animals were again treated with either saline or kisspeptin (100 nmol), i.p., sacrificed after an hour, and gonads were collected. There was no difference among the groups in ovarian (A and C) and uterine (B and D) GPR54 expression. Data expressed as mean ± SEM. C = control; T = kisspeptin treated.