Effects of chronic kisspeptin on GPR54-ir in ovaries and uteri. Saline or kisspeptin (100 nmol i.p) was administered from day 25 to day 50. On day 66, the animals were sacrificed and gonads were collected. GPR54-ir (brown) was detected in the corpus luteum and uterus from controls (A and C) and treated (B and D) animals. A representative section shows that the level of GPR54-ir in the CL and uterus was lower in the kisspeptin treated rats (magnification: 40×).