Representative histological sections, stained with Haematoxylin and Eosin, from caprine ovarian cortical strips cultured in vitro for 7 days with three different concentrations of kisspeptin. (A) Day 0 - uncultured control; (B) Day 7 - cultured control; (C) and (D) Day 7 - with kisspeptin at 1 ng/mL; (E) Day 7 - with kisspeptin at 10 ng/mL; (F) Day 7 - with kisspeptin at 100 ng/mL. Magnification: 40×; scale bar: 100 µm. Follicle viability as well as number of developing follicles were prominent in sections from 1 ng/mL and 10 ng/mL kisspeptin-supplemented strips while wide spread follicle degeneration was evident with 100 µM kisspeptin treatment. While primordial follicles (black arrow), with flat granulosa cells indistinct from ovarian stroma, are prominent in control strips, primary follicles (white arrow), with cuboidal granulosa cells visible around the oocyte, are more in 1 ng/mL and 10 ng/mL kisspeptin-treated strips. Degenerated follicles (black arrow heads) were more in 100 ng/mL kisspeptin-treated strips.