Effect of kisspeptin on caprine primordial follicle development in vitro. (A) Primordial follicles, (B) Intermediate follicles, (C) Primary follicles, (D) Secondary follicles, (E) Degenerated follicles, and (F) Total number of viable follicles. During culture of ovarian cortical strips for 7 days, supplementation of kisspeptin at 1 ng/mL concentration facilitated development of primordial follicles into intermediate, primary and secondary follicles with least number of degenerated follicles. Supplementation of kisspeptin at 10 ng/mL concentration also facilitated development of primordial follicles into primary and secondary follicles with lesser number of degenerated follicles while kisspeptin at 100 ng/mL resulted in degeneration of follicles (statistics: primordial follicles: *vs. all other groups; ɸ vs. day 7 Kiss-10 1 µM and 10 µM; intermediate follicles: *vs. control groups; ɸ vs. day 7 Kiss-10 1 µM and 10 µM; primary follicles: *vs. all other groups; secondary follicles: *vs. control groups; degenerated follicles: *vs. other groups except day 7 Kiss-10 1 µM; ɸ vs. other groups except day 7 Kiss-10 10 µM; Ψ vs. other groups except day 0 control; Φ vs. other groups except day 7 control; θ vs. other groups; total number of viable follicles: *vs. other groups; ɸ vs. other groups; p < 0.05, one-way ANOVA followed by Tukey’s multiple comparison test). Date represented as mean ± SEM; n = 6-8 separate experiments.
pISSN: 2671-4639
