Expression of TRPV1 in Leydig cells of young adult mice. (A) Microphotographs of testis sections. Histological image was obtained by H&E staining. Arrows indicate Leydig cells. (B) TRPV1 immunostaining in testes. Strong green fluorescence intensity shows TRPV1 expression in Leydig cells. White arrows indicate TRPV1 expression in Leydig cells. (C) TUNEL staining. The green signals of TUNEL-labeled apoptotic cells and propidium iodide (PI) stained red signal were merged. The right panel shows the DNase I-treated positive control (PC). Each scale bar represents 50 μm.