Reduction of TRPV1 expression level by testosterone treatment in TM3 cells. (A) Changes in TRPV1 mRNA expression level by testosterone treatment. GAPDH was used as a loading control. (B) TRPV1 protein level changed by testosterone treatment. β-actin was used as a loading control. Each bar represents mean ± SD of three independent experiments. *p < 0.05 compared to the control (solvent treatment). †p < 0.05 compared to the 10 nM testosterone.