pISSN: 2671-4639
eISSN: 2671-4663
pISSN: 2671-4639
eISSN: 2671-4663
The current approaches for camel cumulus-oocyte complex in vitro maturation (IVM)
| IVM type | Main supplement | Effects | In vitro embryo development | References |
|---|---|---|---|---|
| Single phase IVM | 10 % fetal calf serum | Optimizing IVM duration | NA | Kafi et al., 2005;Abdoon et al., 2011 |
| 10 % estrous camel serum | Optimizing ovary storage duration | NA | Wani and Nowshari, 2005 | |
| All-trans retinoic acid | Increased TGFβ expression in cumulus cells and increased MII oocytes | No effect on blastocyst % after PA | Saadeldin et al., 2019 | |
| 10 % follicular fluid | Improved cumulus expansion and MII oocytes | No effect on blastocyst % after PA | Yaqoob et al., 2017 | |
| 15 % fetal calf serum | Optimizing IVM duration | NA | Russo et al., 2014 | |
| 20 ng/mL EGF | Optimizing IVM Improved MII oocytes |
NA | Wani and Wernery, 2010 | |
| PVA | Optimizing IVM Improved MII oocytes |
Improved blastocysts after PA | Moulavi and Hosseini, 2019 | |
| 25 μM melatonin | Improved MII oocytes | Improved blastocysts after IVF | Fathi et al., 2021 | |
| 10 mM caffeine | Improved MII oocytes | Improved blastocysts after IVF | Fathi et al., 2014 | |
| 0.5 mg/mL L-carnitine | Improved MII oocytes | Improved blastocysts after IVF | Fathi and El-Shahat, 2017 | |
| 50 µg/mL of vitamin C | Improved MII oocytes | NA | Al-Malikey and Al-Delemi, 2021 | |
| Biphasic IVM | 50 μM Roscovitine for 24 h | GVBD inhibition | Increased blastocyst formation after PA | Wani and Hong, 2020 |
| 10 µM ROCK inhibitor (Y-27632) for 4 h | Improved cumulus expansion, reduced apoptosis, and increased MII oocytes | NA | Tukur et al., 2020b |
GVBD, Germinal vesicle breakdown; IVF, In vitro fertilization; MII, meiotic II, 1st polar body extrusion; NA, Not applicable; PA, parthenogenetic activation; ROCK, Rho-associated protein kinases.