pISSN: 2671-4639
eISSN: 2671-4663
pISSN: 2671-4639
eISSN: 2671-4663
Summary of the lectins used, including source, sugar specificity, concentration, and inhibitor
| Lectin abbreviations | Source | Sugar specificity |
Concentration(μg/mL) | Inhibitor or eluting sugar |
|---|---|---|---|---|
| N-acetylglucosamine-binding lectins | ||||
| s-WGA | Succinylated-wheat germ agglutinin | GlcNAc | 1.0 × 10-2 | 0.2 M GlcNAc |
| WGA | Wheat germ agglutinin | GlcNAc NeuAc, SA | 1.0 × 10-2 | 0.2 M GlcNAc |
| BSL-Ⅱ | α or β GlcNAc | 4.0 × 10-3 | 0.2 M GlcNAc | |
| DSL | (GlcNAc)2-4 | 2.0 × 10-2 | 0.5 M chitin hydrolysate | |
| LEL | (GlcNAc)2-4 | 1.0 × 10-2 | 0.5 M chitin hydrolysate | |
| STL | (GlcNAc)2-4 | 1.0 × 10-2 | 0.5 M chitin hydrolysate | |
| Mannose-binding lectins | ||||
| ConA | αMan, αGlc, 4GlcNAc | 3.3 × 10-3 | 0.2 M MeαMan/0.2 M MeαGlc | |
| LCA | αMan, αGlc, 4(Fucα1,6)GlcNAc | 4.0 × 10-3 | 0.2 M MeαMan/0.2 M MeαGlc | |
| PSA | αMan, αGlc, 4(Fucα1,6)GlcNAc | 4.0 × 10-3 | 0.2 M MeαMan/0.2 M MeαGlc | |
| Galactose/N-acetylgalactosamine-binding lectins | ||||
| RCA120 | Gal | 2.0 × 10-3 | 0.2 M lactose | |
| BSL-1 | αGal, αGalNAc | 4.0 × 10-3 | 0.2 M GlcNAc | |
| VVA | GalNAc | 4.0 × 10-3 | 0.2 M GlcNAc | |
| DBA | αGalNAc | 1.0 × 10-2 | 0.2 M GlcNAc | |
| SBA | α > βGalNAc | 1.0 × 10-2 | 0.2 M GlcNAc | |
| SJA | βGalNAc | 2.0 × 10-2 | 0.2 M GlcNAc | |
| Jacalin | Galβ3GalNAc | 5.0 × 10-4 | 0.2 M melibiose | |
| PNA | Galβ3GalNAc | 4.0 × 10-3 | 0.2 M βGal | |
| ECL | Galβ3GalNAc | 2.0 × 10-2 | 0.2 M lactose | |
| Complex type N-glycans (complex oligosaccharides)-binding lectins | ||||
| PHA-E | Galβ3GalNAcβ 2 Man α6 (GlcNA cβ4)(GlcNAcβ4Manα 3) Manβ4 | 5.0 × 10-3 | 0.1 M acetic acid | |
| PHA-L | Galβ4GlcNAcβ6 (GluNAcβ2Man α3) Man α3 | 2.5 × 10-3 | 0.1 M acetic acid | |
| Fucose-binding lectin | ||||
| UEA-Ⅰ | αFuc | 2.0 × 10-2 | 0.1 M L-fucose | |
aAcronyms are explained in the text; GlcNAc, N-acetylgalactosamine; NeuAc, N-acetylneuraminic acid; SA, sialic acid; GlcNAc, N-acetylglucosamine; gal, galactose; glc, glucose; Fuc, fucose.
bThe lectin specificities, including sources, preferred sugar specificity, and inhibitors, were modified according to a previous study (Kang et al., 2016).