Changes in cellular apoptosis according to the Mito-TEMPO treatment on vitrified-warmed blastocysts in bovine. (A) Colocalization of DAPI and TUNEL stains at the vitrified-warmed blastocyst stage. The blastocysts were stained for nuclei (blue) and TUNEL-positive cells (green). The white arrows indicate a fragmented DNA of nucleus in blastocysts. (B) The graph indicates the number of nuclei in vitrified-warmed bovine blastocysts. (C) The percentage of TUNEL-positive cells rates depending on the Mito-TEMPO treatment in bovine vitrified-warmed blastocysts are shown. Data are expressed as mean ± SD and were analyzed using a t-test. Differences were considered significant at *p < 0.05 and **p < 0.01.
pISSN: 2671-4639
