Comparison of intracellular and mitochondrial ROS expressions in frozen-thawed bovine blastocysts treated Mito-TEMPO. (A) Expression of intracellular ROS (green fluorescence) in vitrified-warmed bovine blastocysts was detected by DCF-DA and analysis using fluorescence microscope. (B) The graph showed relative ROS expression levels on vitrified-warmed blastocyst in bovine. (C) Representative images of mitochondrial superoxide (red fluorescence) in vitrified-warmed bovine blastocysts detected by Mito-SOX and analysis using image system. (D) Relative superoxide expression levels of vitrified-warmed bovine blastocysts treated Mito-TEMPO. Data are expressed as mean ± SD and were analyzed using a t-test. Differences were considered significant at *p < 0.05.
pISSN: 2671-4639
