Confirmation of mitochondrial activity and localization in the frozen-thawed blastocysts with Mito-TEMPO. (A) Representative images of Mitochondria intensity (orange fluorescence) by MitoTracker Orange staining following the Mito-TEMPO treatment in frozen-thawed blastocysts. Mitochondrial expressions and localization were visualized using a fluorescence microscope. (B) The graph indicated relative fluorescence intensity of mitochondria of bovine frozen-thawed blastocyst. Data are expressed as mean ± SD and were analyzed using a t-test. Differences were considered significant at *p < 0.05.
pISSN: 2671-4639
