Expression patterns of Traf4 during mouse preimplantation development. (A) Transcription levels of Traf4 were measured from the 1‐cell to blastocyst stages using qRT-PCR. Expression levels were normalized to an exogenous control gene (Gfp). (B) Cellular localization of Traf4 were analyzed by immunocytochemistry. Scale bar (50 μm). Error bars represent mean ± standard error.