Effects of Traf4 KD on embryos. (A) Representative images of blastocyst development at 120 h after hCG. (B) Blastocyst development rate. (C) Representative images of defective paracellular sealing in Traf4 KD blastocysts. (D) 4kDa FITC-dextran uptake assay was used for TJs permeability. White arrows indicate collapsed blastocoel.