Table 1

Primer sequences for RT-PCR (F: forward, R: reverse)

Gene	Forward primer	Reverse primer	TM/℃
CAT	5’-GCAGATACCTGTGAACTGTC-3’	5’-GTAGAATGTCCGCACCTGAG-3’	56
SOD	5’-AAAGCGGTGTGCGTGCTGAA-3’	5’-CAGGTCTCCAACATGCCTCT-3’	60
BAX	5’-TGAAGACAGGGGCCTTTTTG-3’	5’-AATTCGCCGGAGACACTCG-3'	58
BCL2	5’-ATGCCTTTGTGGAACTATATGGC-3’	5’-GGTATGCACCCAGAGTGATGC-3'	58
MYF5	5’-ATGCCATCCGCTACATTGAGAG-3’	5’-CCGTCAGAGCAGTTGGAGGT-3'	58
MRF4	5’-CCTCAGCCTCCAGCAGTCTT-3’	5’-TACTTCTCCACCACCTCCTCCA-3'	58
p53	5’-GAACCGCCGACCTATCCTTA-3’	5’-GGCAGGCACAAACACGAAC-3'	58
Beclin-1	5’-ATGGAGGGGTCTAAGGCGTC-3’	5’-TCCTCTCCTGAGTTAGCCTCT-3	58
PCNA	5’-GAT GCC GTC GGG TGA ATT TG-3’	5’-CCA TTG CCA AGC TCT CCA CT-3'	58
MYOG	5’-AGC CAC ACT GAG GGA GAA G-3’	5’-GTT GAG GGA GCT GAG CAA G-3'	58
Ki-67	5’-GAG CTA ACT TGC GCT GAC T-3’	5’-TGT CAC ATT CAA TAC TCC TTC CA-3'	56
GAPDH	5’-TGA AGG TCG GAG TGA ACG GAT TT-3’	5’-CCA TTT GAT GTT GGC GGG AT-3'	58

Journal of Animal Reproduction and Biotechnology 2022;37:17-26 https://doi.org/10.12750/JARB.37.1.17