Primer sequences for RT-PCR (F: forward, R: reverse)
| Gene | Forward primer | Reverse primer | TM/℃ |
|---|---|---|---|
| CAT | 5’-GCAGATACCTGTGAACTGTC-3’ | 5’-GTAGAATGTCCGCACCTGAG-3’ | 56 |
| SOD | 5’-AAAGCGGTGTGCGTGCTGAA-3’ | 5’-CAGGTCTCCAACATGCCTCT-3’ | 60 |
| BAX | 5’-TGAAGACAGGGGCCTTTTTG-3’ | 5’-AATTCGCCGGAGACACTCG-3' | 58 |
| BCL2 | 5’-ATGCCTTTGTGGAACTATATGGC-3’ | 5’-GGTATGCACCCAGAGTGATGC-3' | 58 |
| MYF5 | 5’-ATGCCATCCGCTACATTGAGAG-3’ | 5’-CCGTCAGAGCAGTTGGAGGT-3' | 58 |
| MRF4 | 5’-CCTCAGCCTCCAGCAGTCTT-3’ | 5’-TACTTCTCCACCACCTCCTCCA-3' | 58 |
| p53 | 5’-GAACCGCCGACCTATCCTTA-3’ | 5’-GGCAGGCACAAACACGAAC-3' | 58 |
| Beclin-1 | 5’-ATGGAGGGGTCTAAGGCGTC-3’ | 5’-TCCTCTCCTGAGTTAGCCTCT-3 | 58 |
| PCNA | 5’-GAT GCC GTC GGG TGA ATT TG-3’ | 5’-CCA TTG CCA AGC TCT CCA CT-3' | 58 |
| MYOG | 5’-AGC CAC ACT GAG GGA GAA G-3’ | 5’-GTT GAG GGA GCT GAG CAA G-3' | 58 |
| Ki-67 | 5’-GAG CTA ACT TGC GCT GAC T-3’ | 5’-TGT CAC ATT CAA TAC TCC TTC CA-3' | 56 |
| GAPDH | 5’-TGA AGG TCG GAG TGA ACG GAT TT-3’ | 5’-CCA TTT GAT GTT GGC GGG AT-3' | 58 |
