MMPs activation analysis of mouse vagina tissue. (A, B) In-situ zymography. (A) hcg group, (B) P4 group, (C) Te group. Black arrow is MMPs activation zone. (D) ELISA analysis (bar = 100 μm; magnification: Large picture ×400, The small picture is a 10× magnification of the large picture). *Different letters within the same column represent a significant difference (p < 0.05).