ESCs establishment derived from the parthenogenetic embryos. (A) The morphology of ESCs in each experimental group. Scale bar: 200 μm (B) Cell cycle analysis of the ESCs. ESCs in SrCl2+CB and SrCl2-CB with 2-cell fusion group showed the diploid, while ESCs in the SrCl2-CB group displayed haploid. The mouse fibroblasts were used as a diploid control. (C) The karyotyping of the ESCs in SrCl2-CB group and SrCl2-CB with 2-cell fusion group. The SrCl2-CB group showed haploid and SrCl2-CB with 2-cell fusion group displayed diploid. (D) The rate of ESC establishment from blastocysts and MII oocytes. The rate in SrCl2-CB with the 2-cell fusion group was significantly higher than in the SrCl2-CB group. N, the number of ESCs / the number of blastocysts for the left panel; the number of ESCs / the number of MII oocytes for the right panel. Mean ± S.E.M. *,**, ***, p < 0.05 by ANOVA with Tukey analysis.