Modified Diff Quik stained images of fresh rooster spermatozoa with CBB. After staining with the eosin solution from the Diff Quik stain kits, the smears were dipped into 0.04% (A), 0.08% (C), or 0.3% (D) CBB. The light purple tints of the heads (white arrow in A) and dark purple acrosomes (black arrow in B) showed a more distinct differentiation staining pattern than the classic Diff Quik stains. Black arrowheads in A and D identify unresolved CBB staining with the scale bars respectively (20 μM in A, C, and D; 10 μM in B).
