The percentage of intact acrosome cap in rooster spermatozoa. Three different semen samples were stained using the CBB solution and then compared. The semen smears were prepared within 2 min after centrifugation. The hollow bar was fresh, gray bar was heat stressed, and dark gray was cryopreserved semen. Values represent the mean of three samples ± standard deviation. Superscript indicate significant difference (p < 0.05).