Oligonucleotide primers and PCR cycling conditions
| Genes | GenBank number | Primer sequence | Size (bp) | Temp (℃) | |
|---|---|---|---|---|---|
| Sense (5’ > 3’) | Anti-sense (5’ > 3’) | ||||
| NM_001101.5 | GAGCGAGCATCCCCCAAAGT | TTGGGAGAGGACTGGGCCAT | 166 | 60 | |
| NM_002421 | GAGCAGATGTGGACCATGCCA | CCTGGGCCTGGTTGAAAAGC | 199 | 60 | |
| NM_004530 | TGAGGACTACGACCGCGACAA | CATCTTTCCGTCACTGCGGC | 168 | 60 | |
| BC074815 | CGAGCTGGATACCCAAGAGGC | TTGGGAAAGCCTGGCTCCAT | 175 | 60 | |
| NM_002424 | CCATTCTTTGGGGCTCGCTC | CAGGGTTTGGGTGTGCTTGG | 186 | 60 | |
| NM_004994 | GTTTGGAAACGCAGATGGCG | GCATTGCCGTCCTGGGTGTA | 195 | 60 | |
| NM_002425 | GGCTCTTTCACTCAGCCAAC | CTCAGATCCCGAAGGAACAG | 182 | 60 | |
| NM_002427 | GGCGACTTCTACCCATTTGA | GGTCCTTGGAGTGGTCAAGA | 190 | 60 | |
ACTB, actin beta; MMP, matrix metalloproteinases; Temp, temperature.
