Expression of c-Kit and ANO1 in Leydig cells in testis of young mice. (A) Histological image obtained by H&E staining. Leydig cells around the seminiferous tubules in the center are marked with a green line border. (B) c-Kit and ANO-1 immunostaining in testis. Red fluorescence represents c-Kit and ANO1, which are expressed in Leydig cells. Each scale bar represents 100 μm. NC, negative control.