Expression of c-Kit and ANO1 in TM3 Leydig cells. (A) mRNA expression of c-Kit (PCR product 150 bp) and ANO1 (PCR product 170 bp). The white dotted box indicates c-Kit and ANO1 showing the expected size. GAPDH (PCR product 201 bp) was used as a loading control. (B) Protein expression of c-Kit and ANO1. β-actin was used as a loading control. Each bar represents the mean ± SD of three independent experiments. *p < 0.05 compared to TM3 cells.